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ELISA Kit for C8g

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ELISA Kit for C8g
카다로그 번호   :   SEB970Hu
규격   :   96T
브랜드   :  Cloud-Clone
비고   :  

상세설명

  • Product No.SEB970Hu
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
  • Sample TypeSerum, plasma and other biological fluids
  • Test MethodDouble-antibody Sandwich
  • Assay Length3h
  • Detection Range0.156-10ng/mL
  • SensitivityThe minimum detectable dose of this kit is typically less than 0.072ng/mL.
  • Specificity

    This assay has high sensitivity and excellent specificity for detection of Complement Component 8g (C8g).
    No significant cross-reactivity or interference between Complement Component 8g (C8g) and analogues was observed.

    Recovery

    Matrices listed below were spiked with certain level of recombinant Complement Component 8g (C8g) and the recovery rates were calculated by comparing the measured value to the expected amount of Complement Component 8g (C8g) in samples.

    MatrixRecovery range (%)Average(%)
    serum(n=5)79-9890
    EDTA plasma(n=5)94-10197
    heparin plasma(n=5)78-9894

    Precision

    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Complement Component 8g (C8g) were tested 20 times on one plate, respectively.
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Complement Component 8g (C8g) were tested on 3 different plates, 8 replicates in each plate.
    CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%

    Linearity

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Complement Component 8g (C8g) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

    Sample1:21:41:81:16
    serum(n=5)97-105%80-91%92-102%94-101%
    EDTA plasma(n=5)92-99%91-98%94-105%80-95%
    heparin plasma(n=5)85-94%96-105%96-105%89-101%

    Stability

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
    To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Reagents and materials provided

    ReagentsQuantityReagentsQuantity
    Pre-coated, ready to use 96-well strip plate1Plate sealer for 96 wells4
    Standard2Standard Diluent1×20mL
    Detection Reagent A1×120µLAssay Diluent A1×12mL
    Detection Reagent B1×120µLAssay Diluent B1×12mL
    TMB Substrate1×9mLStop Solution1×6mL
    Wash Buffer (30 × concentrate)1×20mLInstruction manual1

    Assay procedure summary

    1. Prepare all reagents, samples and standards;
    2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
    4. Aspirate and wash 3 times;
    5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
    6. Aspirate and wash 5 times;
    7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
    8. Add 50µL Stop Solution. Read at 450nm immediately. 

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